Study of Antibacterial Activities of Seeds Extract of Fenugreek (Trigonella foenum-graecum)

Background: The antibacterial activity of seeds methanol extract and hot aqueous extract of fenugreek (Trigonella foenum-graecum) showed the presence of activity against four species of bacteria and the methanol extract have higher activity than other extract. Objective: To examine the antimicrobial activities of the crude methanolic extract and hot aqueous extract of seeds of Trigonella foenum-graecum. Patients and Methods: We prepared three different concentrations for all extracts ( 75% ,50%, 25%, v/v ). The study detected antibacterial activity by using well diffusion in agar technique .The study applied on the Four species of bacteria includes :Staphylococcus aureus , Bacillus subtilus, Pseudomonas aeruginosa and Escherichia coli. The study continued from September 2016 to January 2017 in the Laboratory of Biotechnology in College of Science - University of Diyala. Results: The study showed highest antibacterial activity of methanolic extract of seeds of Trigonella foenum –graecum that attained highest zone of inhibition (30 mm) against P. aeruginosa when using (75%) concentration,While attained lowest zone of inhibition (10 mm) against E.coli when using (25%) concentration. Whereas the hot aqueous extract of seeds of Trigonella foenum –graecum that attained highest zone of inhibition (24 mm) against P. aeruginosa when using (75%) concentration, while don't give any effect against bacterial spp. when using (25%)concentration. Conclusion: Antibacterial activity of methanol extract of seeds of Trigonella foenum-graecum was higher than the hot aqueous extract also higher than commercial antibiotic (Amikacin).