Constitutive activation of SYD-2 leads to precocious aggregation and degradation.

(A) HSN synapse formation phenotypes visualized with Airyscan superresolution imaging of endogenous mScarlet-SYD-2 and indicated mutants. SAM1-3Δ and 3E mutants are depleted at HSN synapses. Scale bar, 5 μm. (B) Quantification of HSN intensities in (A). (C) HSN cell body imaging of endogenous mScarlet-SYD-2 and indicated mutants; 10 μm of the proteasome inhibitor bortezomib or DMSO as a control was added to the indicated animals for 4 h prior to imaging. Scale bar, 5 μm. (D) Quantification of SYD-2 puncta in (C). ****, p p S1 Data. HSN, hermaphrodite-specific neuron.