DAPI stains nuclei. PfVP1 was detected by anti-HA and Alexa Fluor 488 anti-mouse secondary antibodies. The food vacuole was detected by anti-PfPlasmepsin II [29] and Alexa Fluor 568 anti-rabbit secondary antibodies. T, trophozoite. S, schizont. Representative images of n = 25 parasites of each stage are shown here. Pearson Correlation Coefficient (0.6303±0.038) of green and red fluorescence was derived from n = 25 parasites. Scale bar, 5 μm. (TIF)</p
<p>a) Live cell ImmunoFluorescence Assay (IFA) with antibodies to HB3var6 (1/50 dilution) tested on ...
A, Immunofluorescence assay (IFA) showing the localization of different mutated PfVP1 proteins tagge...
A, Knockdown experiment starting at the schizont stage by removal of aTc. Giemsa-stained thin blood ...
Representative immuno-EM images of Pf3D7VP2KO-VP1-3HA labeled with anti-HA and gold-conjugated secon...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<p>Schizonts were allowed to release merozoites that invaded fresh erythrocytes in the presence of m...
<p><b>A,</b> Immunofluorescence staining. The cells were fixed and immunostained. Panel (i) phase (i...
<p>Mature parasites at different stages of nuclear division were compared. Each row in panels A and ...
<p>PfCRT was over-expressed as a GFP-fusion protein using an ATet-inducible expression system and tr...
<p>Parasites transformed with GFP- or HA-tagged PfGDV1 were stained with DAPI (DNA stain) and the in...
<p>Sub-cellular localization of PfMA was studied by co-immunostaining with surface protein (<b>A</b>...
<p>A Mature Dd2 schizonts were labeled with rabbit anti-PfRH5 IgG and counterstained with FITC-label...
<p>(A) Cytosolic distribution CpLDH in the extracellular parasite stages, including oocysts, sporozo...
<p>For IFAs, <i>P. falciparum</i> NF54 parasites in the schizont, gametocyte, macrogamete and zygote...
<p>Extracellular sporozoites (A) or HFF monolayers infected with sporozoites for 2–3 hours (B–D), we...
<p>a) Live cell ImmunoFluorescence Assay (IFA) with antibodies to HB3var6 (1/50 dilution) tested on ...
A, Immunofluorescence assay (IFA) showing the localization of different mutated PfVP1 proteins tagge...
A, Knockdown experiment starting at the schizont stage by removal of aTc. Giemsa-stained thin blood ...
Representative immuno-EM images of Pf3D7VP2KO-VP1-3HA labeled with anti-HA and gold-conjugated secon...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<p>Schizonts were allowed to release merozoites that invaded fresh erythrocytes in the presence of m...
<p><b>A,</b> Immunofluorescence staining. The cells were fixed and immunostained. Panel (i) phase (i...
<p>Mature parasites at different stages of nuclear division were compared. Each row in panels A and ...
<p>PfCRT was over-expressed as a GFP-fusion protein using an ATet-inducible expression system and tr...
<p>Parasites transformed with GFP- or HA-tagged PfGDV1 were stained with DAPI (DNA stain) and the in...
<p>Sub-cellular localization of PfMA was studied by co-immunostaining with surface protein (<b>A</b>...
<p>A Mature Dd2 schizonts were labeled with rabbit anti-PfRH5 IgG and counterstained with FITC-label...
<p>(A) Cytosolic distribution CpLDH in the extracellular parasite stages, including oocysts, sporozo...
<p>For IFAs, <i>P. falciparum</i> NF54 parasites in the schizont, gametocyte, macrogamete and zygote...
<p>Extracellular sporozoites (A) or HFF monolayers infected with sporozoites for 2–3 hours (B–D), we...
<p>a) Live cell ImmunoFluorescence Assay (IFA) with antibodies to HB3var6 (1/50 dilution) tested on ...
A, Immunofluorescence assay (IFA) showing the localization of different mutated PfVP1 proteins tagge...
A, Knockdown experiment starting at the schizont stage by removal of aTc. Giemsa-stained thin blood ...