Functional data of G protein coupling.

(A) Concentration-response curves of GPR34 mutants in the Gi-dissociation assay. (B) Expression of GPR34 mutants in HEK293T cells. (C-E) Details of Gαs binding by GPR174 (C), β2AR (D, PDB: 3sn6), and CCKAR (E, PDB: 7ezk). (F) Concentration-response curves of GPR174 mutants in the cAMP accumulation assay. (G) Expression of GPR174 mutants in CHO cells. All data represent mean ± SEM from at least 3 independent experiments. The data used to generate graphs in S6A, S6B, S6F, and S6G are available in S1 Data. (TIF)