Transcriptional remodeling of chromatin architecture at the <i>Tmem131</i> gene.

(A) Pooled Capture Hi-C interaction maps for an approximately 600 kb region around the Tmem131 gene are shown for DN3 and DP cells, above the differential heat map, where DP-enriched interactions are displayed in red and DN3-enriched interactions in blue. The arrows indicate the DN3-specific domain. Positions of genes are shown below the plots. (B) Insulation scores for the same genomic region as in (A), computed at 2 kb resolution with an 80-kb (40 bin) window for DN3 (blue) and DP (red) cells. (C) ChIP-seq profiles (normalized as counts per million reads) for the same genomic region as in (A), for DN3 (blue) and DP (red) cells. Top to bottom: RNA polymerase II, CTCF. Blue stripe indicates DN3-specific domain, with increased insulation score maximum, increased RNA polymerase II loading, and negligible changes in CTCF binding. Source data available in S1 Data. (PDF)