Mutation of <i>SPSL1</i> induces failure of sperm transfer into the spermatheca.

(A-A”) Representative images of spermatheca of A) virgin females, A’) WT females mated with WT males, and A”) WT females mated with SPSL1-mutant males. Scale bars, 1mm. (B) Representative image of spermatheca indicating area used for quantification within red dotted lines. Scale bar, 1mm. (C) The relative grayscale value of spermathecae of virgin females and females for indicated matings. (D) The relative area of spermathecae of virgin females and females for indicated matings. n > 15. Different Uppercase letters, p SPSL1 represents SPSL1 mutants.