Characterization of HFD-fed <i>Pparg</i>^{<i>ΔTbx1</i>} mice.

(A, B) Body weight of female (A, n = 6–7) and male (B, n = 10–12) Ppargf/f and PpargΔTbx1 mice after HFD feeding. (C) Glucose tolerant test of HFD-fed Ppargf/f (n = 8) and PpargΔTbx1 (n = 10) mice (mixed sexes). (D) HFD-fed Ppargf/f (n = 10) and PpargΔTbx1 (n = 10) male mice were fasted overnight, orally gavaged with 4 μg LPS (in 200 μl olive oil), and sera were collected at indicated time for LPS measurement. (E–H) HFD-fed Ppargf/f (10 males, 7 females) and PpargΔTbx1 (8 males, 5 females) mice were fasted and gavaged with 50 μl of 0.1 μg/μl LPS in water per 20 g of body weight. scBAT (E–G) and liver (H) were collected 3 h later for RT-qPCR quantification the expression of Ucp1 (E), Adipoq (F), and inflammatory genes (G, H). Data are presented as mean ± SEM. *, p p t test (E, F) or two-way ANOVA (D). Extended data are listed in S1 Data. HFD, high-fat diet; LPS, lipopolysaccharide; scBAT, supraclavicular brown adipose tissue.