RGMoV icDNA 5’ and 3’ UTR sequence and progeny virus detection.

A–schematic overview of RGMoV genome; B–schematic sequences of newly identified RGMoV icDNA 3’ UTRs in comparison of in GenBank deposited RGMoV complete genome sequences; C–schematic sequences of newly identified RGMoV icDNA 5’ UTR in comparison of in GenBank deposited RGMoV complete genome sequences; D–comparison of Mock and infected with WT RGMoV, icDNA capped RNA transcript, and reinfected with icDNA derived progeny virus A. sativa plant leaves bearing RGMoV classical infection pattern; E–comparison of the minimum free energy RNA structures of old and novel identified 5’ and 3’ UTRs created by RNAfold WebServer [99]; F–transmission electron microscopy analysis of purified virus particles from WT RGMoV, icDNA capped RNA transcript, and reinfected with icDNA derived progeny virus; bar represents 200 nm scale; red and blue marked nucleotides (nt) represents sequences deposited in GenBank and are conserved between isolates; underlined black bold marked nt represent the sequences identified in this research; green marked nt represents single nt changes in deposited sequences.