Comparative transcriptome shows the predominance of female-biased genes.

a) Multidimensional scaling (MDS) plot of 12 samples shows transcriptomic difference of male (blue) and female (red) early prepupal head (n = 6 each). b) MA plot of comparative transcriptome. Among 20565 genes, 89 genes were male-biased and 584 genes were female-biased. Threshold of statistical significance (red dots) is FDR 50% (|logFC| > log2(1.5)). Twenty genes in highlighted zone (yellow) were subjected to functional screening by RNAi.