Effects of silencing <i>PvLLG1</i> on <i>P</i>. <i>vulgaris</i> in non-inoculated or <i>R</i>. <i>tropici</i>-inoculated roots.

(A) Transgenic hairy roots showing expression of red fluorescent protein from nucleotide-scrambled sequence control (SAC) and RNAi:PvLLG1 constructs. (B) PvLLG1 transcript accumulation in P. vulgaris roots expressing SAC or RNAi:PvLLG1 constructs under non-inoculated and R. tropici-inoculated condition. (C) Root hair length in R. tropici-inoculated roots with SAC or RNAi:PvLLG1 constructs. (D) Nodule number at 7, 14, and 21 dpi in R. tropici-inoculated roots with SAC or RNAi:PvLLG1 constructs. (E) Nodule diameter at 7, 14 and 21 dpi in R. tropici-inoculated roots with SAC or RNAi:PvLLG1 constructs. (F) Number of nodules per composite plant with different ranges of diameters at 7, 14 and 21 dpi in R. tropici-inoculated roots with SAC or RNAi:PvLLG1 constructs. (G) Images of root nodules from hairy roots expressing the SAC or RNAi:PvLLG1 constructs at 7, 14 and 21 dpi in R. tropici-inoculated roots. Replications for PvLLG1 transcript accumulation were 15, root hair length were 150, nodule number were 5, and nodule diameter were 150. Different letters or asterisk indicate significant differences among samples according to the Two -Way ANOVA analysis (*p = 0.05, ** p = 0.01, *** p = 0.001) or a pair-wise comparison with a Student´s T- test (p <0.0001), respectively.