NAC decreases the ROS level produced by Ce6.

Fluorescence spectra of 10 μM DCFH-DA in the presence of 10 μM Ce6 (B) with or without (A) 10 mM NAC upon photo-irradiation. The spectra at irradiation time points 0 and 120 min are highlighted in black and red, respectively. (C) Representative fluorescence images of living BY-2 cells after photo-irradiation. Upper: BY-2 cells treated with 10 μM Ce6. Middle: BY-2 cells treated with 10 μM Ce6 and 10 mM NAC. Bottom: BY-2 cells without Ce6 nor NAC served as mock. DIC images of cells and fluorescent images of DCFH-DA are presented. λex = 488 nm, λem = 523 nm. Scale bar, 20 μm. (PDF)