CCR5+ CD4 T_h1 cells populate brain parenchyma.

(A) Flow cytometry plots illustrate frequencies of CD4 and CD8 T cells in blood, CNS tissues, dCLN, and ileum in controls. (B) Contingency plots show % CD4 (solid) and % CD8 (transparent) in each of the control cohort 1 animals (n = 4) assessed. (C) Surface expression of CCR6, CXCR3, and CCR5 on CD95+ CD4 T cells. Pie chart shows preponderance of CXCR3+ CCR5+ (X3 R5) subset in brain relative to spleen. (D) Flow plots and pie chart illustrate expression of α4β1, α4β7, and CCR5 on CD4 Th1 cells. (E) CCR5 / CCR7 distinction in CNS. (F) Contingency plots show % CCR5 (solid) and % CCR7 frequencies (transparent) in each of the control cohort 1 animals (n = 4) assessed. (G) Flow plots show cytokine production following 3-hour stimulation with PMA/Ionomycin in controls (n = 4). (H) Pie Chart and bar graph show proportion of cytokine producing cells across brain (n = 4) and spleen. (n = 4) Significant differences by one tailed Mann Whitney test, *, p< 0.05 in H. Schematics were generated using BioRender.