CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at decreasing ratios (HIDEM/mesoangioblast:PBMC). On day 6 cells were harvested and stained with anti-CD3 antibody and 7AAD, and analysed by flow cytometry. CFSE dilution was analysed on gated CD3+ 7AAD- cells. The percentage of CD3+CFSE dividing cells was calculated for each group and compared to the positive control (P+B), followed by plotting against HIDEM/mesoangioblast:PBMC ratios. Experiments were carried out in duplicates. n=
<div><p>(A) Sorted Treg and memory T-cells were labeled with CFSE and then activated through subopti...
<p>(A) Histograms showing percent proliferation of CFSE-labelled T cells that were polyclonally stim...
<p>A. WT CD4<sup>+</sup>CD62L<sup>hi</sup> T cells were labeled with CFSE and stimulated for 5 days ...
<p>CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) ...
<p>CFSE labelled PBMCs were stimulated with anti-CD3/CD28 beads in the presence of HIDEMs/mesoangiob...
CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads as before in the presence of HIDEMs/mes...
<p>HIDEMs/mesoangioblasts were left untreated or were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml...
HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were ...
<p>HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells we...
A, B. PBMCs (5 × 105) were stimulated with anti-CD3/CD28/CD2-coated beads with or without preculture...
<p><b>A</b>) Proliferation of PBMCs treated with αCD3/αCD28 in the presence of control mIgG1, αOrai1...
<p>(<b>A</b>) The proliferation capacity of MCs throughout <i>in vitro</i> and <i>ex vivo</i> MMT wa...
Time course of polyclonal T cell proliferation. (A) Random donor single buffy coat-derived carboxyfl...
<p>(<b>A)</b> (<b>i</b>) CFSE dilution analysis of labeled OT-II cells cultured with no stimulation ...
For an allogeneic MLR, responder cells from 6 healthy donors were stained with CFSE and mixed with γ...
<div><p>(A) Sorted Treg and memory T-cells were labeled with CFSE and then activated through subopti...
<p>(A) Histograms showing percent proliferation of CFSE-labelled T cells that were polyclonally stim...
<p>A. WT CD4<sup>+</sup>CD62L<sup>hi</sup> T cells were labeled with CFSE and stimulated for 5 days ...
<p>CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) ...
<p>CFSE labelled PBMCs were stimulated with anti-CD3/CD28 beads in the presence of HIDEMs/mesoangiob...
CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads as before in the presence of HIDEMs/mes...
<p>HIDEMs/mesoangioblasts were left untreated or were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml...
HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were ...
<p>HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells we...
A, B. PBMCs (5 × 105) were stimulated with anti-CD3/CD28/CD2-coated beads with or without preculture...
<p><b>A</b>) Proliferation of PBMCs treated with αCD3/αCD28 in the presence of control mIgG1, αOrai1...
<p>(<b>A</b>) The proliferation capacity of MCs throughout <i>in vitro</i> and <i>ex vivo</i> MMT wa...
Time course of polyclonal T cell proliferation. (A) Random donor single buffy coat-derived carboxyfl...
<p>(<b>A)</b> (<b>i</b>) CFSE dilution analysis of labeled OT-II cells cultured with no stimulation ...
For an allogeneic MLR, responder cells from 6 healthy donors were stained with CFSE and mixed with γ...
<div><p>(A) Sorted Treg and memory T-cells were labeled with CFSE and then activated through subopti...
<p>(A) Histograms showing percent proliferation of CFSE-labelled T cells that were polyclonally stim...
<p>A. WT CD4<sup>+</sup>CD62L<sup>hi</sup> T cells were labeled with CFSE and stimulated for 5 days ...