Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method