Add to ORKGSuccessful cryopreservation of hepatocytes would be useful to the pharmaceutical industry and for bioartificial liver support systems. Unfortunately, cryopreservation of hepatocytes in suspension results in low attachment efficiency in culture upon thawing. To circumvent this problem, we have frozen rat hepatocytes as monolayers on collagen substrates. Hepatocytes prepared from male Sprague Dawley rats by collagenase perfusion were cultured (3*106 cells/60mm Petri dish) on collagen films (30mg/cm2) in 2ml Chee's medium containing 5% v/v foetal calf serum (FCS). 24h cultures were frozen in Chee's medium with 10% Dimethyl Sulphoxide and between 0-90% FCS at -708C for 24h
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation has been identified as a necessary barrier to overcome in the production of tissue ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation has been identified as a necessary barrier to overcome in the production of tissue ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
The ability to cryopreserve hepatocytes would be useful both to the pharmaceutical industry and for ...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
To improve post-thaw attachment efficiency in culture exhibited by hepatocytes frozen in suspension,...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Little work in the literature focuses on the cryopreservation of primary hepatocytes as monolayer cu...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation of primary hepatocyte monolayers may provide a means of long-term storage of the ce...
Cryopreservation has been identified as a necessary barrier to overcome in the production of tissue ...