The effect of dietary ingredients manipulation at various life stages of giant freshwater prawn, macrobrachium rosenbergii production

The present study was carried out determine the effect of dietary ingredients manipulation at various life stages of giant freshwater prawn, M. rosenbergii productions. Four feeding experiments were conducted to investigate the effects of dietary ingredients on the growth performances, survival, feed utilization efficiency and whole body composition of giant freshwater prawn, Macrobrachium rosenbergii. In experiment 1, five isoproteic (40 %) and isolipidic (10 %) diets with fishmeal (FM), shrimp meal (SHM), squid meal (SQM), bivalve meal (BVM) and a mixture of SHM, SQM and BVM (MIX) were fed to triplicate groups of broodstock and cultured in a recirculation water system. The mean initial wet weight of females and males broodstock were 30 g and 70 g, respectively and stocked at 5 prawns/tank (1 male : 4 females). The prawn was fed at 3 % of body weight (SW), three times daily (8 am, 12 pm and 5 pm) for 180 days. The fecundities of prawn fed MIX (1449.52 ± 64.15 egg/g female) and BVM (1308.53 ± 40.41 egg/g female) were significant higher (P 0.05) difference among treatments. The results showed that up to 75 % of fish meal can be replaced with PBM without adversely affecting the growth performance of PL. In experiment 4, isoproteic (40 %) and isolipidic (10 %) diets with 0 % (control diet), 75 % (75PBM), 80 % (80PBM), 85 % (85PBM), 90 % (90PBM), 95 % (95PBM) and 100 % (100PBM) PBM replacing FM protein were fed to triplicate groups of juvenile prawn (average initial weight: 3.77 ± 0.03 g) and cultured in a recirculation water system at 16 juveniles/ tank (dimension: 49 x 49 x 31 cm; volume 70 L). The prawn was fed three times daily (9 am, 1 pm and 5 pm) at 5 % of their body weight for 40 days. FW, WG and SGR were the highest in lO0PBM (9.66 ± 0.21 g, 160.64 ± 7 .38 % and 2.06 ± 0.07 %/day, respectively) followed by 95PBM, 80PBM, 85PBM, 75PBM, 90PBM and 0PBM. The FCR during the feeding trials ranged from 1.50 to 2.20. The survival of juvenile prawn (83.33-89.58 %) did not show any significant different (P> 0.05) among treatments. The result showed that the utilization of PBM is improved in larger M. rosenbergii. In conclusion, manipulation of dietary ingredients such as locally available marine protein (shrimp, squid and bivalve) and PBM can be successfully used in the production of M. rosenbergii at different life stages without negatively affecting their performances in terms of growth performance, survival, feed utilization efficiency and whole body composition