Nuclear domains are membraneless organelles found in the nuclei of eukaryotic cells. The B-body is a nuclear domain that accumulates the protein Bruno (Bru) and exists in the developing flight muscles in Drosophila. We used the B-body and Bru as a model to study formation of nuclear domains. Specifically, we searched for the amino acid sequences that make Bru accumulate in B-bodies. We used genetic engineering to create various Bru mutants, which were subsequently tested in flies. Nuclear distribution of mutants was analyzed by cryosectioning, immunostaining, and fluorescence microscopy. Three RRM domains determine Bru binding to RNA. We determined that all RRMs must be intact for Bru to interact with the RNA scaffold inside the B-body. The...
The cell nucleus contains a large number of membrane-less bodies that play important roles in the sp...
1 The nuclear lamina is a filamentous network that underlies the nuclear envelope. Lamina components...
Copyright © 2011, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Re...
The eukaryotic nucleus is a busy place with a high concentration of proteins performing diverse func...
Despite the lack of internal membranes, the cell nucleus is neatly organized into various kinds of d...
Nuclear bodies (NBs) (e.g., the nucleolus, nuclear speckles, and others) are membraneless compartmen...
Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal tissues whe...
An assembly process involving sequential recruitment of components and hierarchical dependency drive...
Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that per...
Electronic publication date given is for the publisher's version found on Elsevier's webpage, and no...
textRNA recognition motifs (RRMs) are a ubiquitous class of proteins which bind RNA in a sequence-sp...
Background: Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal...
SummaryBackgroundThe shape of a nucleus depends on the nuclear lamina, which is tightly associated w...
BACKGROUND: The shape of a nucleus depends on the nuclear lamina, which is tightly associated with t...
Background: The shape of a nucleus depends on the nuclear lamina, which is tightly associated with t...
The cell nucleus contains a large number of membrane-less bodies that play important roles in the sp...
1 The nuclear lamina is a filamentous network that underlies the nuclear envelope. Lamina components...
Copyright © 2011, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Re...
The eukaryotic nucleus is a busy place with a high concentration of proteins performing diverse func...
Despite the lack of internal membranes, the cell nucleus is neatly organized into various kinds of d...
Nuclear bodies (NBs) (e.g., the nucleolus, nuclear speckles, and others) are membraneless compartmen...
Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal tissues whe...
An assembly process involving sequential recruitment of components and hierarchical dependency drive...
Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that per...
Electronic publication date given is for the publisher's version found on Elsevier's webpage, and no...
textRNA recognition motifs (RRMs) are a ubiquitous class of proteins which bind RNA in a sequence-sp...
Background: Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal...
SummaryBackgroundThe shape of a nucleus depends on the nuclear lamina, which is tightly associated w...
BACKGROUND: The shape of a nucleus depends on the nuclear lamina, which is tightly associated with t...
Background: The shape of a nucleus depends on the nuclear lamina, which is tightly associated with t...
The cell nucleus contains a large number of membrane-less bodies that play important roles in the sp...
1 The nuclear lamina is a filamentous network that underlies the nuclear envelope. Lamina components...
Copyright © 2011, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Re...