Kejadian Pertama Infeksi Zucchini yellow mosaic virus pada Tanaman Mentimun di Padang, Sumatera Barat

Tanaman mentimun varietas lokal Padang merupakan tanaman sayuran unggulan bagi petani perkotaan karena umur panen yang pendek. Hasil survei menemukan adanya gejala bercak kuning dan klorosis dengan tulang daun hijau. Gejala ini mirip dengan infeksi Papaya ring spot virus (PRSV) yang menginfeksi mentimun di Jawa dan Zucchini yellow mosaic virus (ZYMV) yang menginfeksi mentimun pada umumnya. Deteksi virus dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan primer spesifik PRSV dan primer universal Potyvirus, perunutan dan analisis DNA. RT-PCR menggunakan primer spesifik PRSV DNA target tidak menghasilkan pita DNA, namun pita DNA dengan ukuran ±320 pb berhasil teramplifikasi dengan primer universal Potyvirus. Analisis sikuen nukleotida menunjukkan bahwa similaritas virus tertinggi sebesar 93.8% dan 93.5%, berturut-turut dengan ZYMV isolat Singapura dan India. Infeksi ZYMV merupakan kejadian pertama pada tanaman mentimun di Padang, Sumatera Barat.The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra Cucumber Local Padang variety is considered as a superior vegetable crop by urban farmers due to its early harvest time. Yellow spots and chlorosis symptoms was found on cucumber plants during field surveys conducted recently. These symptoms are similar to Papaya ring spot virus (PRSV) infection on cucumbers in Java, and common symptoms of Zucchini yellow mosaic virus (ZYMV) infection on cucurbits. Detection of the causal virus was carried out by the reverse transcription polymerase chain reaction (RT-PCR) using PRSV specific primer and universal primers of Potyvirus, DNA sequencing and analysis. DNA target was not amplified by RT-PCR using PRSV specific primer, but DNA fragment of ±320 bp was successfully amplified using Potyvirus universal primer. The nucleotide sequences analysis showed highest similarity of 93.7% and 93.5% to the ZYMV isolates from Singapore and India, respectively. This is the first report on the occurrence of ZYMV infection on cucumber plants in Padang, West Sumatra