ANALYSIS OF VOLATILE COMPOUNDS IN THE PROCESS OF COUNERFEITING HONEY USING MAPLE SYRUP, SUGAR AND FLOWER POLLEN : diplom athesis

U ovom radu analiziran je kemijski sastav hlapljivih spojeva u procesu patvorenja meda. Za analizu su uzeti uzorci cvjetne peludi, meda, javorovog sirupa, saharozne probe te smjesa cvjetne peludi i javorovog sirupa i smjesa cvjetne peludi i saharozne probe. Med i cvjetna pelud koji su bili osnova za istraživanje patvorenja uzeti su s podruĉja Slavonskog Broda. Za patvorenje je korišten komercijalni javorov sirup i komercijalni šećer pomoću kojeg se u laboratoriju napravila saharozna proba. Hlapljivi spojevi izolirani su metodom mikroekstrakcije vršnih para na krutoj fazi ( HS-SPME) korištenjem sivog vlakna s ovojnicom divinilbenzen/karboksen/polidimetilsiloksan (DVB/CAR/PDMS). Dobiveni uzorci analizirani su vezanom tehnikom plinska kromatografija-spektrometrija masa (GC-MS). Analizom patvorenog meda naĉinjenog od smjese cvjetne peludi i javorovog sirupa te smjese cvjetne peludi i saharozne probe detektiran je niz spojeva koji su analizom detektirani i u medu.In this study, the chemical composition of volatile compounds in the process of honey adulteration was analysed. Samples of flower pollen, honey, maple syrup, sucrose and a mixture of flower pollen and maple syrup and a mixture of flower pollen and sucrose were taken for the analysis. The honey and flower pollen, which formed the basis for the adulteration investigation, came from the Slavonski Brod region. Commercial maple syrup and commercial sugar were used for adulteration, and a sucrose sample was prepared in the laboratory. The volatile compounds were isolated by the solidphase microextraction method (HS-SPME), using a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coated fibre. The obtained samples were analyzed by gas chromatography-mass spectrometry (GC-MS). By analyzing the adulterated honey made from a mixture of flower pollen and maple syrup, as well as a mixture of flower pollen and sucrose, a series of compounds were detected that were also found in the honey alaysis