Comparative analysis of TMS-EEG signal using different approaches in healthy subjects

openThe integration of transcranial magnetic stimulation with electroencephalography (TMS-EEG) represents a useful non-invasive approach to assess cortical excitability, plasticity and intra-cortical connectivity in humans in physiological and pathological conditions. However, biological and environmental noise sources can contaminate the TMS-evoked potentials (TEPs). Therefore, signal preprocessing represents a fundamental step in the analysis of these potentials and is critical to remove artefactual components while preserving the physiological brain activity. The objective of the present study is to evaluate the effects of different signal processing pipelines, (namely Leodori et al., Rogasch et al., Mutanen et al.) applied on TEPs recorded in five healthy volunteers after TMS stimulation of the primary motor cortex (M1) of the dominant hemisphere. These pipelines were used and compared to remove artifacts and improve the quality of the recorded signals, laying the foundation for subsequent analyses. Various algorithms, such as Independent Component Analysis (ICA), SOUND, and SSP-SIR, were used in each pipeline. Furthermore, after signal preprocessing, current localization was performed to map the TMS-induced neural activation in the cortex. This methodology provided valuable information on the spatial distribution of activity and further validated the effectiveness of the signal cleaning pipelines. Comparing the effects of the different pipelines on the same dataset, we observed considerable variability in how the pipelines affect various signal characteristics. We observed significant differences in the effects on signal amplitude and in the identification and characterisation of peaks of interest, i.e., P30, N45, P60, N100, P180. The identification and characteristics of these peaks showed variability, especially with regard to the early peaks, which reflect the cortical excitability of the stimulated area and are the more affected by biological and stimulation-related artifacts. Despite these differences, the topographies and source localisation, which are the most informative and useful in reconstructing signal dynamics, were consistent and reliable between the different pipelines considered. The results suggest that the existing methodologies for analysing TEPs produce different effects on the data, but are all capable of reproducing the dynamics of the signal and its components. Future studies evaluating different signal preprocessing methods in larger populations are needed to determine an appropriate workflow that can be shared through the scientific community, in order to make the results obtained in different centres comparable