Temperature-Sensitive Nanocarbon Hydrogel for Photothermal Therapy of Tumors

Wanlin Tan,1,2 Chen Sijie,1,2 Yan Xu,1,2 Mingyu Chen,1,2 Haiqin Liao,1,2 Chengcheng Niu1,2 1Department of Ultrasound Diagnosis, the Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China; 2Research Center of Ultrasonography, the Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of ChinaCorrespondence: Chengcheng Niu, Email niuchengcheng@csu.edu.cnBackground: Intelligent hydrogels continue to encounter formidable obstacles in the field of cancer treatment. A wide variety of hydrogel materials have been designed for diverse purposes, but materials with satisfactory therapeutic effects are still urgently needed.Methods: Here, we prepared an injectable hydrogel by means of physical crosslinking. Carbon nanoparticle suspension injection (CNSI), a sentinel lymph node imaging agent that has been widely used in the clinic, with sodium β-glycerophosphate (β-GP) were added to a temperature-sensitive chitosan (CS) hydrogel (CS/GP@CN) as an agent for photothermal therapy (PTT). After evaluating the rheological, morphological, and structural properties of the hydrogel, we used 4T1 mouse breast cancer cells and B16 melanoma cells to assess its in vitro properties. Then, we intratumorally injected the hydrogel into BALB/c tumor-bearing mice to assess the in vivo PTT effect, antitumor immune response and the number of lung metastases.Results: Surprisingly, this nanocarbon hydrogel called CS/GP@CN hydrogel not only had good biocompatibility and a great PTT effect under 808nm laser irradiation but also facilitated the maturation of dendritic cells to stimulate the antitumor immune response and had an extraordinary antimetastatic effect in the lungs.Discussion: Overall, this innovative temperature-sensitive nanocarbon hydrogel, which exists in a liquid state at room temperature and transforms to a gel at 37 °C, is an outstanding local delivery platform with tremendous PTT potential and broad clinical application prospects.Keywords: temperature-sensitive chitosan, carbon nanoparticle suspension, photothermal therapy, hydrogels, antitumor immune respons