LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF CEFEPIME AND TAZOBACTAM IN DOG PLASMA

Kiran R. Patil
Sukanta K. Naik
Vineet S. Zope
Rajesh P. Chavan
Ravindra D. Yeole

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Publication date

September 2018

Publisher

PGDesign / Universidade Federal do Rio Grande do Sul

Journal

Drug Analytical Research

Abstract

A high performance liquid chromatography (HPLC) method with tandem mass spectrometric detection (MS/MS) has been developed and validated for the simultaneous quantification of cefepime and tazobactam in dog plasma. The method was developed on amide column with isocratic elution. The developed method is simple and economic in terms of sample preparation. The method is specific, sensitive, accurate, precise and robust. The method was successfully applied for pre-clinical pharmaco-kinetic studies in dogs. The Tmax was found to be 0.5 hr, the mean Cmax and AUC(0-12) displayed dose proportionate response