The neuronal activity has a unique genetic signature that can be used for personal identification and authentication. Continuous authentication of individuals is required in the field involving high security, such as military services, intelligence organizations and secret agencies. Electroencephalogram (EEG) based authentication method is favorable due to its uniqueness and the fact that it can be used even when the person is unconscious. In this study, we investigated the number of samples per subject required to reliably develop a biometric trait authentication system. Initially, we extracted the background EEG signals from the publicly available Temple University Hospital (TUH) database. A total of 46 statistical and frequency domain fe...