T cell changes across the MCC disease trajectory.

(A) T cell compartment subtypes are represented as a UMAP. (B) Expression of GAPDH in four T cell subtypes. (C) Expression of the exhaustion-related markers. (D) Expression levels of various subtype markers in T cells. Red dots indicate high expression and blue dots indicate low expression. (E) Correlation between proliferation marker expression and GAPDH expression in C3 cluster cells. (F) Exhaustion score calculated based on expression levels of exhaustion markers. “**” indicates that the T-test p-value is less than 0.05. (G) Differentiation trajectory of CD8+ T cells in MCC, with each color-coded for clusters (top) and pseudotime (bottom). (H) Expression of the common immune checkpoints. Red indicates high expression and blue indicates low expression. (I) The expression of some genes in the validation cohort. (J) The predicted proportion of C3 cluster cells in the validation cohort. (K) Number of DEGs between RespD376 and ARD614 in CD8+ T cells. (L) Venn diagrams represent 82 DEGs that were up-regulated in RespD376 and down-regulated in ARD614. (M) Functional enrichment modules of 82 DEGs, colored by module type.