Abundance and distribution in microorganism species from T0-20 and T20-40.

(A) box plot of abundance and (B) Venn graph of distribution of total microorganism species (C) relative abundance of different microorganism species among groups. The box plot was drawn according to the result of significant differences in the abundance of the different species between T0-20 and T20-40. Based on OTUs results obtained by clustering, common and unique OTUs between T0-20 and T20-40 were analyzed to form Venn graph. The numbers of circles and overlaps represent the number of OTUs shared between T0-20 and T20-40. According to the species annotation results, the top 10 species with the largest abundance in each sample at each taxonomic level (Phylum, Class, Order, Family, Genus and Species) were selected to generate the columnar accumulation chart of relative abundance of species. ‘Others’ represents the sum of the relative abundances of all other species except those 10 species. The data was obtained based on the OTUs results using soil samples, and each soil sample includes triplicates.