(related to Fig 3). ST2 is highly expressed on adaptive immune subsets, but this expression is dispensable for host survival.

(A) Scheme for CD8 T cell depletion during intracranial WNV-E218A infection, created using Biorender.com and exported via an academic subscription belonging to AO. (B) Peripheral blood counts 6 DPI with WNV-E218A to confirm CD8 depletion. (C) Survival analysis of mice with CD8 T cell depletion and WNV-E218A intracranial infection. (D) Weight change in mice with CD8 T cell depletion and control IgG injection following WNV-E218A intracranial infection. (E) Representative FACs plots and gating strategy for indicated cell types from brain homogenate 7 days post intracranial WNV-E218A infection. (F) Representative FACs plots of ST2 expression on indicated cell types from B6/J and Il1rl1–/–brain homogenate 7 days post intracranial WNV-E218A infection. (G) Quantification of ST2 excision on splenic and liver Tregs of indicated genotype one-month post tamoxifen treatment. (H) Weight change in mice with Treg ST2 deletion following intracranial infection with ZIKV-Dakar. (I) Scheme and cell number from peripheral blood of indicated immune cells following IL-2 complex treatment and WNV-E218A infection at 9 DPI. (J) gMFI of CD25 and GITR on peripheral blood Tregs at 9 DPI with WNV-E218A and IL-2 complex treatment. (K) Survival analysis of mice with control or IL-2 complex treatment following intracranial infection with WNV-E218A. (L) Weight change in mice with control or IL-2 complex treatment after intracranial infection with WNV-E218A. Data are representative of 2 pooled independent experiments (error bars, SEM). * p (TIF)