MAYV strains in IFNαR-/- mice.

Four 13-week-old IFNαR-/- mice per group received a subcutaneous right footpad injection of 104 PFU of MAYVBeAr505411, MAYVCH, MAYVGuyane, MAYVTRVL, MAYVUruma, or UNAVMAC150. Mice were bled at 1 dpi for peak serum viremia and body weights and footpad swelling were recorded daily until animals were euthanized due to excessive loss of body weight. Serum titer of infectious virus measured by plaque-forming units per mL (PFU/mL) is log-transformed and shown in (A). Statistical analysis for comparison of viral titers was completed using a one-way ANOVA with log transformed data, where **** p 0.05. The Kaplan-Meier survival curve is shown in (B) for the four-day monitoring period until mice succumbed to infection. A Kruskal-Wallis test was used to compare survival data for the groups of mice and the only significant comparison was survival of MAYVTRVL-infected compared to UNAVMAC150 -infected mice, p C) and percent change from starting weight (%) is shown in (D). Error bars are SEM when included. (EPS)