Evaluation of MAYV strain pathogenesis in C57BL/6 mice.

Five 4-week-old female C57BL/6 mice were infected with 104 plaque forming units (PFU) with one of five MAYV strains or UNAV via a right foot pad 20μL injection. Blood was collected for serum isolation at 2 days post-infection (dpi) and tissues were harvested at 5 dpi. Titers of infectious virus in serum at 2 dpi are shown in (A) and viral RNA (vRNA) levels in tissues were quantified (B-J). Data points are mean with SEM error bars for n = 5 per group, measuring three replicates of log-transformed data. Serum was tittered on Vero cells by limiting dilution plaque assays and vRNA in tissues was measured in triplicate by qRT-PCR (vRNA copies per well were normalized to the RsP17 house keeping gene.) The LOD in (A) was 100 PFU/mL with undetectable samples graphed as 50 PFU/mL. The LOD in (B-J) was 50 vRNA copies per well or per 200μL homogenate. Statistical analysis was completed using a one-way ANOVA with log-transformed data, where **** p 0.05.