Longitudinal peripheral blood and lymphoid tissue cell phenotype activation of monocytes, dendritic cells, and NK cells following MAYV infection.

Macaque PBMC from -28, 0–5, 7, and 10 dpi (A-F) and lymphocytes isolated from three lymphoid tissues either one month prior to infection or 10 dpi (G-L) were analyzed for cell phenotype using flow cytometry. Changes in the longitudinal frequency of both total and activated (CD169+) classical monocytes (A), non-classical monocytes (B), intermediate monocytes (C), NK cells (D), myeloid dendritic cells (E), and plasmacytoid dendritic cells (F) are quantified. Comparison of the frequencies of intermediate, classical, and non-classical monocyte phenotype activation at pre- or post-infection are quantified for axillary (Ax) LN (G), mesenteric (Mes) LN (H), and spleen tissues (I). Frequency of NK cell and dendritic cell activation comparing pre- and post-infection is shown for axillary LN (J), mesenteric LN (K) and spleen tissues (L). Lines represent mean frequencies of the three animals and error bars represent the standard error of the mean. Longitudinal changes in total or activated (CD169+) cells in the peripheral blood (A-F) were analyzed using paired t tests where baseline (d0) was compared to the peak of the phenotype between 2 or 4 dpi; for this analysis, **** p 0.05. Statistical analyses for comparisons of baseline vs. 10 dpi cell phenotype frequencies in the lymphoid tissues (G-L) were completed using two-tailed paired t tests; only significant comparisons are shown, all other comparisons yielded ns p values > 0.05.