Differentially expressed genes (DEGs) in MoDCs-P and MODCs-NP at 4 h post-infection.

(A) Volcano plot of RNA-seq data. MoDCs were infected with pathogenic leptospires (MoDCs-P, including serovar Pomona, PO, and a clinical isolate, CI) and non-pathogenic leptospires (MoDCs-NP, including serovar Patoc, PA, and Ranarum, RA) at 4 h post-infection. The dotted vertical lines bound the minimal fold-change for the most differentially expressed genes. Red dots represent upregulated genes, and blue dots represent downregulated genes (adjusted P-value 1). The data represent results from three donors. (B) Relative RT-qPCR analysis of PTGS2 expression in MoDCs infected with leptospires for 4 h, calculated relative to uninfected MoDCs (Un). Each bar represents the mean ± SD of three donors. Conditions with distinct letters are significantly different (P < 0.05), whereas those with shared letters are not significantly different.