Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against T. brucei lysate, mouse brain lysates, or BSA. The total IgG ASCs is also included.

Protein-specific antibody responses to vaccine antigens.

M. Jeff Bergen (365461)
Chien-Hsiung Pan (365462)
Catherine E. Greer (365463)
Harold S. Legg (365464)
John M. Polo (365465)
Diane E. Griffin (306426)

February 2013

Serum IgG specific for MV H (A), F (B) and N (C) proteins over a 125-day tim...

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtained from first and second stage HAT patients.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtai...

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.</p

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against T. brucei lysate, mouse brain lysates upon treatment with LTBR-Ig.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against ...

Supporting data for S7E Fig—Comparison of IgM antibody secreting cells (ASCs) in the meninges against T. brucei or mouse brain lysates. Total IgM ASCs are also included.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7E Fig—Comparison of IgM antibody secreting cells (ASCs) in the meninges agains...

Detection of antibody secreting cells (ASCs).

Kar Muthumani (504713)
Megan C. Wise (504714)
Kate E. Broderick (396225)
Natalie Hutnick (252194)
Jonathan Goodman (504715)
Seleeke Flingai (504716)
Jian Yan (49839)
Chaoran B. Bian (504717)
Janess Mendoza (504718)
Colleen Tingey (504719)
Christine Wilson (504720)
Krzysztof Wojtak (504721)
Niranjan Y. Sardesai (49844)
David B. Weiner (49846)

December 2013

Groups of mice (n = 4) were immunized with the multi-clade constructs. (A) 96-well plates ...

The murine meninges contain autoreactive IgG+ ASCs during chronic T. brucei infection.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

(A) Representative ELISpot images depicting mouse brain-specific IgG+ ASCs from naïve and infected m...

Supporting data for S6A Fig—Comparison of antibody secreting cells in the meninges and splenocytes against mouse brain lysate.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S6A Fig—Comparison of antibody secreting cells in the meninges and splenocytes a...

Supporting data for Fig 7E—Serum IgM and IgG ELISA test against mouse brain lysates from naïve and infected animals.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7E—Serum IgM and IgG ELISA test against mouse brain lysates from naïve and i...

HBs-specific antibody secreting cells (ASC) in splenocytes of immune mice as measured by ELISPOT.

Guohong Ge (148889)
Shixia Wang (126712)
Yaping Han (148894)
Chunhua Zhang (148898)
Shan Lu (50076)
Zuhu Huang (126716)

July 2012

Mice were immunized with HBs-L or HBs-L(T) DNA vaccine, or with empty DNA vector as indicated. Th...

Supporting data for S7B Fig—Clinical scoring of mice treated with LTBR-Ig blocking antibody of IgG2a isotype control.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7B Fig—Clinical scoring of mice treated with LTBR-Ig blocking antibody of IgG2a...

Detection of HA tri-stalk specific memory B cells.

I-Na Lu (4586797)
Anna Kirsteina (4586782)
Sophie Farinelle (321117)
Stéphanie Willieme (5798306)
Kaspars Tars (1334421)
Claude P. Muller (106167)
Andris Kazaks (1474987)

September 2018

(A) ELISPOT assay workflow. (B) Representative pictures of spot-forming IgG1- or IgG2a-secreting cel...

Chronic T. brucei infection induces demyelination in the CNS.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

(A) Left: Representative ELISpot images depicting mouse brain-specific IgG+ ASCs from naïve and infe...

LTβR signalling blockade results in uncontrolled parasitaemia and IgM+ B cell accumulation in the meninges.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Parasitaemia (A) and clinical scoring (B) of T. brucei-infected mice treated with the LTβR-Ig fusion...

Supporting data for S7A Fig—Parasitaemia of mice treated with LTBR-Ig blocking antibody of IgG2a isotype control (<5.4 falls below detection limit).

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7A Fig—Parasitaemia of mice treated with LTBR-Ig blocking antibody of IgG2a iso...

Protein-specific antibody responses to vaccine antigens.

M. Jeff Bergen (365461)
Chien-Hsiung Pan (365462)
Catherine E. Greer (365463)
Harold S. Legg (365464)
John M. Polo (365465)
Diane E. Griffin (306426)

February 2013

Serum IgG specific for MV H (A), F (B) and N (C) proteins over a 125-day tim...

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtained from first and second stage HAT patients.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtai...

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.</p

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against T. brucei lysate, mouse brain lysates upon treatment with LTBR-Ig.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against ...

Supporting data for S7E Fig—Comparison of IgM antibody secreting cells (ASCs) in the meninges against T. brucei or mouse brain lysates. Total IgM ASCs are also included.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7E Fig—Comparison of IgM antibody secreting cells (ASCs) in the meninges agains...

Detection of antibody secreting cells (ASCs).

Kar Muthumani (504713)
Megan C. Wise (504714)
Kate E. Broderick (396225)
Natalie Hutnick (252194)
Jonathan Goodman (504715)
Seleeke Flingai (504716)
Jian Yan (49839)
Chaoran B. Bian (504717)
Janess Mendoza (504718)
Colleen Tingey (504719)
Christine Wilson (504720)
Krzysztof Wojtak (504721)
Niranjan Y. Sardesai (49844)
David B. Weiner (49846)

December 2013

Groups of mice (n = 4) were immunized with the multi-clade constructs. (A) 96-well plates ...

The murine meninges contain autoreactive IgG+ ASCs during chronic T. brucei infection.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

(A) Representative ELISpot images depicting mouse brain-specific IgG+ ASCs from naïve and infected m...

Supporting data for S6A Fig—Comparison of antibody secreting cells in the meninges and splenocytes against mouse brain lysate.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S6A Fig—Comparison of antibody secreting cells in the meninges and splenocytes a...

Supporting data for Fig 7E—Serum IgM and IgG ELISA test against mouse brain lysates from naïve and infected animals.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7E—Serum IgM and IgG ELISA test against mouse brain lysates from naïve and i...

HBs-specific antibody secreting cells (ASC) in splenocytes of immune mice as measured by ELISPOT.

Guohong Ge (148889)
Shixia Wang (126712)
Yaping Han (148894)
Chunhua Zhang (148898)
Shan Lu (50076)
Zuhu Huang (126716)

July 2012

Mice were immunized with HBs-L or HBs-L(T) DNA vaccine, or with empty DNA vector as indicated. Th...

Supporting data for S7B Fig—Clinical scoring of mice treated with LTBR-Ig blocking antibody of IgG2a isotype control.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7B Fig—Clinical scoring of mice treated with LTBR-Ig blocking antibody of IgG2a...

Detection of HA tri-stalk specific memory B cells.

I-Na Lu (4586797)
Anna Kirsteina (4586782)
Sophie Farinelle (321117)
Stéphanie Willieme (5798306)
Kaspars Tars (1334421)
Claude P. Muller (106167)
Andris Kazaks (1474987)

September 2018

(A) ELISPOT assay workflow. (B) Representative pictures of spot-forming IgG1- or IgG2a-secreting cel...

Chronic T. brucei infection induces demyelination in the CNS.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

(A) Left: Representative ELISpot images depicting mouse brain-specific IgG+ ASCs from naïve and infe...

LTβR signalling blockade results in uncontrolled parasitaemia and IgM+ B cell accumulation in the meninges.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Parasitaemia (A) and clinical scoring (B) of T. brucei-infected mice treated with the LTβR-Ig fusion...

Supporting data for S7A Fig—Parasitaemia of mice treated with LTBR-Ig blocking antibody of IgG2a isotype control (<5.4 falls below detection limit).

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for S7A Fig—Parasitaemia of mice treated with LTBR-Ig blocking antibody of IgG2a iso...

Protein-specific antibody responses to vaccine antigens.

M. Jeff Bergen (365461)
Chien-Hsiung Pan (365462)
Catherine E. Greer (365463)
Harold S. Legg (365464)
John M. Polo (365465)
Diane E. Griffin (306426)

February 2013

Serum IgG specific for MV H (A), F (B) and N (C) proteins over a 125-day tim...

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtained from first and second stage HAT patients.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 7G—IgM and IgG ELISA test against human brain lysates from CSF samples obtai...

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.

Juan F. Quintana (8044493)
Matthew C. Sinton (11056182)
Praveena Chandrasegaran (17420073)
Lalit Kumar Dubey (5758280)
John Ogunsola (17420076)
Moumen Al Samman (17420079)
Michael Haley (7646750)
Gail McConnell (2180448)
Nono-Raymond Kuispond Swar (17420082)
Dieudonné Mumba Ngoyi (7889651)
David Bending (17420085)
Luis de Lecea (711657)
Annette MacLeod (12779)
Neil A. Mabbott (7361273)

November 2023

Supporting data for Fig 10G—Mouse serum IgG against mouse brain lysates upon suramin treatment.</p

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against <i>T</i>. <i>brucei</i> lysate, mouse brain lysates, or BSA. The total IgG ASCs is also included.

Abstract

Extracted data

Supporting data for Fig 7B—Quantification of IgG antibody secreting cells (ASCs) by ELISpot against <i>T</i>. <i>brucei</i> lysate, mouse brain lysates, or BSA. The total IgG ASCs is also included.

Abstract

Extracted data

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