Heterogeneity of meningeal MNPs during chronic <i>T</i>. <i>brucei</i> infection.

(A) UMAP of 10,760 high-quality MNPs from naïve (n = 54 cells) and infected meninges (n = 10,706 cells). (B) Expression level of top genes defining different populations of meningeal MNPs. (C) Frequency plot depicting the relative abundance of the 5 MNPs subclusters identified in the murine meninges during T. brucei infection. Supporting data in S7 Data file. (D) Cell–cell interaction network via MCH-II signalling axis. (E) Violin plot depicting the expression level of H2-D1 and H2-Ab1, 2 of the most up-regulated MHC-II associated genes within the myeloid compartment. (F) Cell–cell interaction network via Cxcl signalling axis. (G) Representative flow cytometry analysis and quantification (H) of CD11b+ F4/80High and F4/80Low myeloid cell populations, as well as MHC-II+ myeloid cells, in the murine meninges in response to T. brucei infection. Data in all panels are representative from 2 independent experiments and is expressed as mean ± SD (n = 5 mice/experimental group). Data points indicate biological replicates for each panel. A parametric T test was employed to assess significance between experimental groups. A p value S8 Data file. MNP, mononuclear phagocyte; UMAP, uniform manifold approximation and projection.