Additional file 1 of Mutant p53 murine oviductal epithelial cells induce progression of high-grade serous carcinoma and are most sensitive to simvastatin therapy in vitro and in vivo

Additional file 1: Supplemental Figure 1. Confirmation of p53, PAX2 and PAX8 expression in murine oviductal epithelial (OVE) cells, human fallopian tube epithelial cells, spontaneously transformed murine ovarian surface epithelial cells and metastatic ascites cells. A. Protein was collected from OVE and human fallopian tube epithelial cells (n = 4/group) and western blotting was performed to confirm p53 expression in p53R175H mutant OVE cells (OVE4MUT, OVE16MUT. Wild-type p53 cells (OVE4, OVE16, FT237) and Trp53 knockout OVE cells (OVE4KO, OVE16KO) have no p53 expression, confirming normal wild-type function or Trp53 gene knockout. The transcription factors PAX2 and PAX8 are characteristic to fallopian tube development, but are not expressed in the ovary. Immunofluorescence staining confirms (B) PAX2 and (C) PAX8 staining in all murine OVE and human fallopian tube epithelial cells. Additionally, murine ID8 and 28-2 cells, which are of ovarian surface epithelial origin, have minimal to no expression of PAX2 and PAX8. Bars represent mean ± SEM (*p < 0.05, ****p < 0.0001). Scale bars: 50μm. Abbreviations: WT wild-type; MUT p53 mutant; KO Trp53 knockout