Introduction of TEM and its application

A transmission electron microscope (TEM) has the merit of being higher resolution of images than an optical microscope. The TEM has been applied to some fine morphological studies, especially to the fields of cell biology and medical science. This paper reports principle and general structure of TEM and the main features of Hitachi H-7600 TEM which one of the most useful TEM in this field for some convenient functions, such as an auto-focus function, a personal condition setting, a specimen position memory. Application of the H-7600 TEM for three-dimensional reconstruction of a protein by electron tomography is also discussed