Morphological and molecular analyses of Bipteria lusitanica n. sp in wild white seabream, Diplodus sargus (Linnaeus, 1758) in Portugal

PubMed ID: 29748714WOS: 000435595600005The present study records the presence of Bipteria lusitanica n. sp. in wild white seabream, Diplodus sargus (Linnaeus, 1758) off the Portuguese coast. Myxosporean parasites were found in the interstitial tissue of kidney and free in urinary bladder of D. sargus with a 33.3% of prevalence of infection. Myxospores were triangular or inversely pyramidal in shape and anteriorly wider in frontal view, measuring 11.2 +/- 1.0 mu m in length, 12.6 +/- 0.9 mu m in width, and 11.6 +/- 0.4 mu m in thickness. The sinuous sutural line was parallel to the axis that connects the center of the two polar capsules. Spore valves were smooth without ridges. Wing-like appendages extended from the posterior part of each valve in sutural view, measuring 3.9 +/- 1.1 mu m in length. Spherical polar capsules measured 4.4 +/- 0.2 mu m in diameter and the polar filaments were helical arranged in 5-6 turns. Molecular data showed that this parasite clusters within species of the Sinuolineidae Shulman, 1959 family, and they all infect the urinary bladder. Using molecular and morphological characterization, we were able to identify this parasite as a novel species of the genus Bipteria Kovaleva, Zubtchenko, and Krasin, 1983.This research work was partially supported by the Structured Program of R;D;I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources, reference NORTE-01-0145-FEDER-000035, within the Research Line "INSEAFOOD Innovation and valorization of seafood products: meeting local challenges and opportunities," within the R;D Institution CIIMAR (Interdisciplinary Centre of Marine and Environmental Research), supported by the Northern Regional Operational Programme (NORTE2020), through the European Regional Development Fund (ERDF). This research was also partially supported by the Strategic Funding MD/Multi/04423/2013 through national funds provided by FCT - Foundation for Science and Technology and European Regional Development Fund (ERDF), in the framework of the program PT2020. C. Sirin was funded by the 2214-PhD student research fellowship program of the Scientific and Technological Research Council of Turkey (TUBITAK).Structured Program of RDI INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources within the Research Line "INSEAFOOD Innovation and valorization of seafood products: meeting local challenges and opportunities," wi [NORTE-01-0145-FEDER-000035]; FCT Foundation for Science and Technology and European Regional Development Fund (ERDF), in the framework of the program PT2020 [MD/Multi/04423/2013]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2214