There has been an increase in the flow of freight vehicles commuting on Brazilian highways. Then, special attention to the structural performance of bridges regarding the fatigue in beams is needed. Brazil has neither normative metrology to study real data flow of vehicles, nor specific fatigue load train models and coefficients to the analysis and design of road bridges. The same load train that is used for general dimensioning, TB 450, is used for the fatigue verification. Hence, this work aims to verify if the current TB 450 is representative of the freight heavy vehicles with 2 to 9 axles concerning the effects of fatigue in the longitudinal reinforcement of beams of theoretical reinforced concrete bridges with two, three, and five beam...