In vitro characterization of BM-MSCs and SHED.

A) Morphology and proliferation of BM-MSCs and SHED. Cells showed a spindle-like fibrous morphology. B) Surface markers of BM-MSCs and SHED using Flow cytometry analyses. The expression of the positive markers, CD73, CD90 and CD105, was present in more than 95% of the cells, while that of the negative markers, CD34, CD45 and HLA-DR, was present in less than 2% of the cells. C) Multi-differentiation potential of cultured BM-MSCs and SHED. Osteogenic differentiation for 21 days followed by staining with Alizarin Red S. Adipogenic induction and differentiation for 21 days followed by staining with Oil Red O. Scale bars A-E = 200 μm, F = 50.