NMR-based metabolomics of the culture supernatants of epimastigotes grown for 7 days.

(A) Principal component analysis score plot and (B) loading plot of four independent experiments (S6 Table). (C) Box plots showing the peak intensity of secreted molecules in parasite supernatants after 7 days of culture. (D) Heatmap representing the fold change of secreted molecules. The fold change was calculated by metabolite intensity measured in parasite supernatants relative to metabolite intensity found in cell-free medium. (E) Box plots show the peak intensity of metabolites which exhibited low levels compared to cell-free medium (consumed molecules). The results show mean ± SE. Dashed line is the peak intensity of the molecule found in low (6.6 mM) Glc cell-free medium (black line) and high (12 mM) Glc cell-free medium (gray line). a qb q(S7 Table); *q(S8 Table); analyzed by the Two-way ANOVA with FDR approach calculated by the Bejamini, Krieger and Yekutiele method. q-value1 = secreted molecules to the medium; q-value<0.05 with fold change<1 = consumed molecules from the medium.