Efficient Algorithm for Primer Design

PCR is one of the most popular technique that enable amplify specific region of the genome. It allows performing variety of analyses and application including DNA cloning for sequencing; functional analysis of genes; the diagnosis of hereditary diseases. Primer design is one of the most fundamental in PCR-based methods. Many different parameters need to be taken into account. There is many commercial program, some of them are online available, for the primer design. In this paper we present an algorithm which is not located in analyzing large sequence but for infrequent users