Influence of salinity and temperature on the growth, productivity, photosynthetic activity and intracellular ROS of two marine microalgae and cyanobacteria

Global Climate Change could change physical parameters in oceans, such as salinity and temperature. The impact of such changes in phytoplankton has not been well stated yet. In this study the effect of combination of three levels of temperature (20, 23, and 26 °C), and three levels of salinity (33, 36, and 39) on growth of a mixture co-cultivation of three common species from phytoplankton (one cyanobacteria, Synechococcus sp., and two microalgae, Chaetoceros gracilis, and Rhodomonas baltica), is monitored by flow cytometry under controlled cultivation conditions in a 96 h study. Chlorophyll content, enzymes activities and oxidative stress were also measured. Results demonstrate that cultures of Synechococcus sp. Exhibited a high growth at the highest temperature chosen in this study (26 °C) combined with the three selected salinity levels 33, 36, and 39. Nevertheless, Chaetoceros gracilis grew very slowly with the combination of high temperature (39 °C) and all salinities, while Rhodomonas baltica did not grow at temperatures higher than 23 °C. Maximum dry biomass and ash-free dry weight for the microalgal mixture were reached at salinity of 39 and temperature of 20 °C, the but highest chlorophyll fluorescence values were found at 30 salinity and 20 °C, decreasing as salinity and temperature increased.his study was funded by EU-NextGeneration. Infrastructure was founded by FEDER and MCIN/AEI/10.13039/501100011033 (Project EQC2019-005953-P). Dr. Rajaa Kholssi benefits from a Margarita Salas research grant. Microalgae were cultured by the use of infrastructure acquired by a FEDER project (EQC2019-005953-P: Multi-species zoo and phytoplankton culture system for research at the ICMAN-CSIC).Peer reviewe