Evolutionary rate and genetic load in an emblematic Mediterranean tree following an ancient and prolonged population collapse

15 Pág.Severe bottlenecks significantly diminish the amount of genetic diversity and the speed at which it accumulates (i.e., evolutionary rate). They further compromise the efficiency of natural selection to eliminate deleterious variants, which may reach fixation in the surviving populations. Consequently, expanding and adapting to new environments may pose a significant challenge when strong bottlenecks result in genetic pauperization. Herein, we surveyed the patterns of nucleotide diversity, molecular adaptation and genetic load across 177 gene-loci in a circum-Mediterranean conifer (Pinus pinea L.) that represents one of the most extreme cases of genetic pauperization in widespread outbreeding taxa. We found very little genetic variation in both hypervariable nuclear microsatellites (SSRs) and gene-loci, which translated into genetic diversity estimates one order of magnitude lower than those previously reported for pines. Such values were consistent with a strong population decline that began some ~1 Ma. Comparisons with the related and parapatric maritime pine (Pinus pinaster Ait.) revealed reduced rates of adaptive evolution (α and ωa ) and a significant accumulation of genetic load. It is unlikely that these are the result from differences in mutation rate or linkage disequilibrium between the two species; instead they are the presumable outcome of contrasting demographic histories affecting both the speed at which these taxa accumulate genetic diversity, and the global efficacy of selection. Future studies, and programs for conservation and management, should thus start testing for the effects of genetic load on fitness, and integrating such effects into predictive models.This research was supported by funding from EVOLTREE (www.evoltree.eu), a former EU Network of Excellence, and currently a European Research Group facilitated by the European Forest Institute (EFI), and B4EST, a project funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No 773383. JPJ-C was supported by the “Dirección General de Asuntos del Personal Académico” (DGAPA-UNAM) through a PASPA fellowship, for a sabbatical leave during which this study was elaborated.Peer reviewe