This paper reports the results of the documentation and non-invasive analysis performed on a wall painting representing the Crucifixion and attributed to Francesco d’Antonio Zacchi, also known as Balletta, an important 15th century artist operating in Viterbo (Italy). The wall painting is located in a chapel on the right wall of the church of Santa Maria Nova, one of the oldest churches in Viterbo with a great religious relevance for the local parishioners. The documentation and the diagnostic analysis were performed in 2014 on the occasion of the last conservative intervention promoted by Rotary Club and founded by the Bank of Viterbo. The intervention was aimed at cleaning the wall paintings by removing the old restoration materials and t...