A Multimethodological Characterization of Cannabis sativa L. Inflorescences from Seven Dioecious Cultivars Grown in Italy: The Effect of Different Harvesting Stages

Spano Mattia
Di Matteo Giacomo
Ingallina Cinzia
Botta Bruno
Quaglio Deborah
Ghirga Francesca
Balducci Silvia
Cammarone Silvia
Campiglia Enio
Giusti Anna Maria
Vinci Giuliana
Rapa Mattia
Ciano Salvatore
Mannina Luisa
Sobolev Anatoly

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Publication date

January 2021

DOI

10.3390/molecules26102912

Journal

Molecules

Abstract

The chemical profile of the female inflorescence extracts from seven Cannabis sativa L. dioecious cultivars (Carmagnola, Fibranova, Eletta Campana, Antal, Tiborszallasi, Kompolti, and Tisza) was monitored at three harvesting stages (4, 14, and 30 September), reaching from the be ginning of flowering to end of flowering/beginning of seed formation, using untargeted nuclear magnetic resonance (NMR) and targeted (ultra-high-performance liquid chromatography (UHPLC) and spectrophotometry) analyses. The tetrahydrocannabinol content was always below the legal limits (<0.6%) in all the analyzed samples. The NMR metabolite profile (sugars, organic acids, amino acids, and minor compounds) subjected to principal components analysis (PCA) showed a s...