A single genomic locus associated with piebalding across many breeds.

(A) Whole-genome pFST comparisons of piebald birds to non-piebald birds. Dashed red line indicates 5% threshold for genome-wide significance. (B) Plot of genotypes within the candidate region identified by pFST. Each row shows an individual bird, and each vertical line a SNP position colored by genotype relative to the reference genome. Approximate locations of genes are illustrated at top. Shading on the left-hand side indicates birds with shared fixed haplotypes, the approximate span of these haplotypes is illustrated by gray blocks on the bottom of the plot. Hom Ref, homozygous reference allele; Het, heterozygous; Hom Alt, homozygous alternate (non-reference) allele.