Using PBS to identify signal enrichment in ChIP-seq datasets.

(A) Schematic of the PBS method. (B) Signal tracks (i), called peaks (ii) and PBS values (iii) for several histone modifications at a sample locus in tissue from esophagus. This example was chosen for demonstration as the locus has signal from both narrow and broad histone marks in this tissue. (C) Heatmap of a collection of 28 H3K27ac datasets from four different tissue categories showing signal at the CDKN2A locus. Highlighted boxes demonstrate enrichment of H3K27ac signal proximal to IFNA8 in central nervous system tissues (i), CDKN2A in all tissue categories (ii), and LINC01239 in gastrointestinal and cardiovascular tissues (iii).