Microbial growth, intracellular ATP, and thermogenesis assay of selected kinase knockouts.

(A) Average strain growth (OD600) curves over time. (B) Average strain intracellular ATP concentration (mM) over time. (C) Average strain heat flow (mW) curves over time. Curve with error bars is included in S11 Fig. ΔarcB–red, ΔglnL–blue, ΔyccC–orange, and wild-type–black. Significance calculated for each strain against wild type by ANOVA. Error bars: ± SD. *p p p p S1 Data.