Analysis of clusters obtained from subclustering cluster #0 (Astrocyte_1; homeostatic astrocytes).

(A) UMAP of the subclusters obtained from the subclustering of Astrocyte_1 (cluster #0). (B) Act79B expression levels plotted on the same UMAP. (C) Maximum projection of astrocyte(R86E01)>nls::GFP adult optic lobe where Act79B expression (magenta) was detected by in situ HCR in a sparse subset of GFP (green) positive astrocyte nuclei of the medulla, lobula, and lobula plate. Dashed lines outline the neuropils and scale bar is 20 μm. (D) Quantification of Act79B positive astrocyte nuclei (i.e., GFP positive nuclei) from (C) approximately 11% of astrocyte nuclei were positive for Act79B in vivo. The percentage of positive nuclei within each indicated region was quantified, showing Act79B was preferentially expressed in astrocytes of the lateral and proximal medulla, lobula, and lobula plate (Chi-squared test, p S5 and S7 Data files and on GitHub at https://github.com/VilFernandesLab/2022_DrosophilaGlialAtlas. HCR, hybridization chain reaction; UMAP, uniform manifold approximation and projection.