FIGURE 4 from Statistical Assessment of Drug Synergy from <i>In Vivo</i> Combination Studies Using Mouse Tumor Models

invivoSyn detects synergy with high statistical power and low FDR under regular tumor growth patterns. A and B, Simulations were conducted to obtain empirical statistical power to detect Bliss synergy with respect to the number of mice n in a group, assuming equal number of mice in all four groups, under regular tumor growth where tumors in a group either grow or shrink with variations in GR. TV is measured twice a week for 3 weeks (on days 0, 4, 7, 10, 14, 18, 21) assuming that tumor DT is 7 days, and the TV cutoff is set to 3,000 mm3. C, Simulation results at four DT values (DT = 3, 5, 7, 10 days) with all other simulation parameters same as in A, showing that power increases with respect to DT for a given n value for both weak and strong synergies, while for nil synergy, the power is always low. D, Simulation results under five tumor volume cutoffs (500, 1,000, 1,500, 2,000, 2,500 mm3) for mouse euthanasia with DT = 5 days, showing that power is not strongly affected by TV cutoff.