Incoherent imaging using continuous wave ultrasound. A preliminary study using bovine intervertebral discs

As an object rotates with respect to a stationary planar ultrasonic beam, the scattering centres within the object return echoes that are Doppler-shifted in frequency by amounts depending on the velocities of the individual scatterers. The backscattered echo amplitude at any particular frequency is the line integral of the scattered radiation at the cross-range corresponding to that frequency. The amplitude as a function of frequency can be interpreted as a tomographic projection. A tomographic reconstruction algorithm can produce an image of the distribution of scattering centres in the insonified object from these projections. This paper describes the development and characterisation of a microscanner to investigate the approach of using continuous wave ultrasound for three-dimensional cross-sectional imaging. The results of preliminary tissue investigation, conducted using bovine coccygeal intervertebral discs, are described. The radial imaging resolution improves as the Doppler frequency resolution improves but the circumferential resolution degrades proportionally. As the number of projections increases, there is a finite increase in image quality. Two- and three-dimensional images of the intervertebral disc reveal an alternate light and dark banding pattern that is characteristic of the laminar structure of the annulus fibrosus