Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrelated cell growth inhibitors, p16 and alternative reading frame (ARF). In fibroblasts, both proteins are implicated in cellular senescence, a key barrier to tumor development. The p16 coding sequence is more often mutated in melanoma families than is the ARF sequence. To investigate the role of p16 in melanocytes, we assessed aspects of growth, apoptosis, and immortalization in melanocytes cultured from two melanoma patients, both of whom had two inactive p16 alleles but functional ARF. Methods: Growth and senescence were evaluated by cumulative population-doubling curves, and apoptosis by terminal deoxytransferase labeling. Expression of p53 ...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Aim: The Mitogen-Activated Protein Kinase (MAPK) pathway and the AKT pathway regulate cell growth, s...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: Oncogene-induced senescence is considered to act as a potent barrier to cell transformat...
The p16INK4a-Rb tumour suppressor pathway is required for the initiation and maintenance of cellular...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Oncogene-induced senescence acts as a barrier against tumour formation and has been implicated as th...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Oncogene-induced senescence acts as a barrier against tumor formation and has been implicated as the...
Activated oncogenes restrict cell proliferation and transformation by triggering a DNA damage-depend...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Aim: The Mitogen-Activated Protein Kinase (MAPK) pathway and the AKT pathway regulate cell growth, s...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrel...
Background: Oncogene-induced senescence is considered to act as a potent barrier to cell transformat...
The p16INK4a-Rb tumour suppressor pathway is required for the initiation and maintenance of cellular...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Oncogene-induced senescence acts as a barrier against tumour formation and has been implicated as th...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Malignant melanomas often arise from nevi, which result from initial oncogene-induced hyperprolifera...
Oncogene-induced senescence acts as a barrier against tumor formation and has been implicated as the...
Activated oncogenes restrict cell proliferation and transformation by triggering a DNA damage-depend...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Human diploid fibroblasts (HDFs) can be grown in culture for a finite number of population doublings...
Aim: The Mitogen-Activated Protein Kinase (MAPK) pathway and the AKT pathway regulate cell growth, s...